Real-time imaging of DNA-streptavidin complex formation in solution using a high-speed atomic force microscope

Mime Kobayashi, Koji Sumitomo, Keiichi Torimitsu

研究成果: Article査読

47 被引用数 (Scopus)

抄録

The direct observation of individual molecules in action is required for a better understanding of the mechanisms of biological reactions. We used a high-speed atomic force microscope (AFM) in solution to visualize short DNA fragments in motion. The technique represents a new approach in analyzing molecular interactions, and it allowed us to observe real-time images of biotinylated DNA binding to/dissociating from streptavidin protein. Our results show that high-speed AFMs have the potential to reveal the mechanisms of molecular interactions, which cannot be determined by analyzing the average value of mass reactions.

本文言語English
ページ(範囲)184-190
ページ数7
ジャーナルUltramicroscopy
107
2-3
DOI
出版ステータスPublished - 2007 2 1
外部発表はい

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Instrumentation

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