Reaction intermediates in the heme degradation reaction by HutZ from: Vibrio cholerae

Takeshi Uchida, Yukari Sekine, Nobuhiko Dojun, Ariel Lewis-Ballester, Izumi Ishigami, Toshitaka Matsui, Syun Ru Yeh, Koichiro Ishimori

研究成果: Article査読

8 被引用数 (Scopus)

抄録

HutZ is a heme-degrading enzyme in Vibrio cholerae. It converts heme to biliverdin via verdoheme, suggesting that it follows the same reaction mechanism as that of mammalian heme oxygenase. However, none of the key intermediates have been identified. In this study, we applied steady-state and time-resolved UV-vis absorption and resonance Raman spectroscopy to study the reaction of the heme-HutZ complex with H2O2 or ascorbic acid. We characterized three intermediates: oxyferrous heme, meso-hydroxyheme, and verdoheme complexes. Our data support the view that HutZ degrades heme in a manner similar to mammalian heme oxygenase, despite their low sequence and structural homology.

本文言語English
ページ(範囲)8104-8109
ページ数6
ジャーナルDalton Transactions
46
25
DOI
出版ステータスPublished - 2017

ASJC Scopus subject areas

  • Inorganic Chemistry

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