Ratiometric fluorescence detection of pyrimidine/purine transversion by using a 2-amino-1,8-naphthyridine derivative

Hiroyuki Satake, Seiichi Nishizawa, Norio Teramae

研究成果: Article査読

16 被引用数 (Scopus)

抄録

A new class of abasic site-binding fluorescence ligands, Naph-NBD in which 7-nitrobenzo-2-oxa-1,3-diazole (NBD) is connected to 2-amino-7-methyl-1,8- naphthyridine (Naph) by a propylene linker, is presented for the ratiometric assay for SNPs typing. In solutions buffered to pH 7.0 (I = 0.11 M, at 5°C), Naph-NBD is found to selectively recognize pyrimidine bases over purine bases opposite the abasic site in DNA duplexes (K11/M1: T, 8.1 x 106; C, 2.5 x 106; G, 0.33 x 106; A, 0.27 x 106). The binding of Naph-NBD is accompanied by significant quenching of the fluorescence from the naphthyridine moiety (λmax;, 409 nm), while the emission from the NBD (λmax;, 544 nm) is relatively unaffected. Such a fluorescence response of Naph-NBD allows the emission ratio detection of pyrimidine/purine transversion. 2006

本文言語English
ページ(範囲)195-197
ページ数3
ジャーナルanalytical sciences
22
2
DOI
出版ステータスPublished - 2006 2月

ASJC Scopus subject areas

  • 分析化学

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