Rat gastric prepepsinogen: In Vitro synthesis and partial amino-terminal signal sequence

Yoshikazu Ichihara, Kazuhiro Sogawa, Kenji Takahashi

    研究成果: Article査読

    7 被引用数 (Scopus)

    抄録

    The major translation product of rat gastric mucosa RNA in a wheat germ cell-free system was identified as prepepsinogen by electrophoretic analysis of its immuno-precipitate on sodium dodecyl sulfate (SDS)-polyacrylamide gels and amino-terminal sequence determination. The translation product containing radioactive amino acids, purified by SDS-polyacrylamide gel electrophoresis, was shown to have an amino-terminal extension peptide comprising 16 amino acid residues. A partial amino acid sequence of this extension peptide is as follows: Met-X-X-Met-Val-Val-X-Leu-Leu-X-Leu-X-Leu-Leu-X-X-pepsinogen.

    本文言語English
    ページ(範囲)603-606
    ページ数4
    ジャーナルJournal of biochemistry
    92
    2
    DOI
    出版ステータスPublished - 1982 1 1

    ASJC Scopus subject areas

    • 生化学
    • 分子生物学

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