Rapid detection of homologously integrated DNA fragments and accurate quantitation of their copy number in transgenic Aspergillus oryzae by PCR

Munechika Honda, Toshitaka Minetoki, Katsuya Gomi, Masayuki Machida

研究成果: Article査読

2 被引用数 (Scopus)

抄録

A simple and rapid method for the analysis of artificially introduced DNA fragments has been developed using competitive PCR and long and accurate PCR. The locus and the copy number of the DNA fragments in each Aspergillus oryzae transformant could be detected more rapidly and accurately by this method than by the conventional Southern hybridization method.

本文言語English
ページ(範囲)577-579
ページ数3
ジャーナルJournal of Bioscience and Bioengineering
90
5
DOI
出版ステータスPublished - 2000

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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