Rapid detection and identification of mycobacteria by combined method of polymerase chain reaction and hybridization protection assay

A. Hashimoto, H. Koga, S. Kohno, Y. Miyazaki, M. Kaku, K. Hara

研究成果: Article査読

11 被引用数 (Scopus)

抄録

A new method for the rapid detection and identification of mycobacteria, combining polymerase chain reaction (PCR) with DNA probe, was developed. The mycobacterial 16S rRNA encoding gene was amplified by PCR, and the PCR product was identified by hybridization protection assay using acridinium ester labelled DNA probe. The optimum temperature for hybridization of PCR product and DNA probe was estimated as 55°C. The specificity of the method was excellent in the detection and identification of Mycobacterium tuberculosis and Mycobacterium avium-intracellulare complex (MAC). The detection limit was 10 fg DNA for M. tuberculosis, and 100 fg DNA for MAC. Results of preliminary clinical investigation of this method using 207 clinical specimens demonstrated an efficient detection and identification of M. tuberculosis and MAC. These results indicate that the new combined method may be a useful test for the rapid detection and identification of M. tuberculosis and MAC in clinical specimens.

本文言語English
ページ(範囲)71-77
ページ数7
ジャーナルJournal of Infection
33
2
DOI
出版ステータスPublished - 1996
外部発表はい

ASJC Scopus subject areas

  • 微生物学(医療)
  • 感染症

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