Rapid and specific screening assay for kras oncogene mutation by a novel gene amplification method

Akira Kumasaka, Naoyuki Matsumoto, Shotaro Mukae, Taiichi Kitano, Hiroyasu Noguchi, Hidero Ohki, Kazuo Komiyama, Tomohiro Ando

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Background: Epidermal growth factor receptor (EGFR) is a target of molecular therapeutics for colorectal cancer. However, mutation of Kirsten rat sarcoma viral oncogene homolog (KRAS) gene at codons 12 and 13 attenuates the therapeutic effect of anti-EGFR therapies. Therefore, the detection of KRAS gene mutation is important for therapeutic decision-making. Materials and Methods: KRAS gene mutation at codons 12 (c.34G>T, c.35G>C, c.35G>A) and 13 (c.38G>A) in six cancer cell lines were investigated. A loop-mediated isothermal amplification-based procedure was developed that employed peptide nucleic acid to suppress amplification of the wild-type allele. Results: This mutation-oriented gene-amplification procedure can amplify the DNA fragment of the KRAS gene with codon 12 and codon 13 mutation within 30 min. Moreover, boiled cells can work as template resources. Conclusion: This newly developed procedure can be useful for patient stratification for anti-EGFR therapies.

本文言語English
ページ(範囲)1571-1579
ページ数9
ジャーナルAnticancer research
36
4
出版ステータスPublished - 2016 4
外部発表はい

ASJC Scopus subject areas

  • 腫瘍学
  • 癌研究

フィンガープリント

「Rapid and specific screening assay for kras oncogene mutation by a novel gene amplification method」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル