Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species

Go Suzuki, Yuka Ogaki, Nozomi Hokimoto, Lin Xiao, Akie Kikuchi-Taura, Chiaki Harada, Ryozo Okayama, Asami Tsuru, Misa Onishi, Naoko Saito, Geum Sook Do, Sun Hee Lee, Takuro Ito, Akira Kanno, Maki Yamamoto, Yasuhiko Mukai

    研究成果: Article査読

    11 被引用数 (Scopus)

    抄録

    BAC FISH (fluorescence in situ hybridization using bacterial artificial chromosome probes) is a useful cytogenetic technique for physical mapping, chromosome marker screening, and comparative genomics. As a large genomic fragment with repetitive sequences is inserted in each BAC clone, random BAC FISH without adding competitive DNA can unveil complex chromosome organization of the repetitive elements in plants. Here we performed the comparative analysis of the random BAC FISH in monocot plants including species having small chromosomes (rice and asparagus) and those having large chromosomes (hexaploid wheat, onion, and spider lily) in order to understand a whole view of the repetitive element organization in Poales and Asparagales monocots. More unique and less dense dispersed signals of BAC FISH were observed in species with smaller chromosomes in both the Poales and Asparagales species. In the case of large-chromosome species, 75-85% of the BAC clones were detected as dispersed repetitive FISH signals along entire chromosomes. The BAC FISH of Lycoris did not even show localized repetitive patterns (e.g., centromeric localization) of signals.

    本文言語English
    ページ(範囲)621-628
    ページ数8
    ジャーナルPlant Cell Reports
    31
    4
    DOI
    出版ステータスPublished - 2012 4

    ASJC Scopus subject areas

    • Agronomy and Crop Science
    • Plant Science

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