Raman imaging microscopy for quantitative analysis of biological samples

研究成果: Chapter

抄録

Raman imaging microscopy is a powerful tool for label-free imaging of biological samples. It has the advantage of measuring the spatial distribution of endogenous proteins and lipids in cells, as well as obtaining chemical information on these endogenous molecules, such as hydrogen bonding and electrostatic interactions. However, because Raman intensity is very weak compared with fluorescence intensity, obtaining a reliable Raman image requires fast acquisition of a Raman image and rejection of background fluorescence. In this chapter, we describe the procedure for obtaining images of the Raman band of interest using a multipoint technique, which is the fast acquisition method for obtaining an image.

本文言語English
ホスト出版物のタイトルAdvances in Experimental Medicine and Biology
出版社Springer New York LLC
ページ163-172
ページ数10
1035
DOI
出版ステータスPublished - 2017

出版物シリーズ

名前Advances in Experimental Medicine and Biology
1035
ISSN(印刷版)0065-2598
ISSN(電子版)2214-8019

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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