Radiotracer studies of cystic fibrosis transmembrane conductance regulator expressed in Xenopus oocytes

T. Ohrui, W. Skach, M. Thompson, J. Matsumoto-Pon, C. Calayag, J. H. Widdicombe

    研究成果: Article査読

    10 被引用数 (Scopus)

    抄録

    We measured fluxes of radiotracers in Xenopus oocytes expressing the cystic fibrosis transmembrane conductance regulator (CFTR). Addition of adenosine 3',5'-cyclic monophosphate (cAMP)-elevating agents [forskolin and 3-isobutyl-1-methylxanthine (I/F)] led to large increases in uptake of 36Cl, 125I, and 82Br into oocytes expressing wild-type CFTR or ΔF508 CFTR but not sham-injected oocytes. I/F also stimulated halide efflux from CFTR and ΔF508 oocytes in the sequence Cl > Br > I. cAMP-induced increases in 36Cl efflux from ΔF508 oocytes were ~20% of those in CFTR oocytes. Increases in halide efflux were blocked by diphenylamine-2-carboxylic acid but not by 4,4'-diisothiocyanostilbene-2,2'-disulfonic acid. The phorbol ester, phorbol 12-myristate 13-acetate, also stimulated 36Cl efflux from CFTR oocytes. ATP uptakes into CFTR and sham oocytes were similar, and both were reduced by I/F. However, ATP uptake into I/F-treated CFTR oocytes was slightly greater (~40%) than into I/F-treated sham oocytes. Urea uptake into CFTR and sham oocytes was similar and in both cases was increased by I/F. However, the I/F-induced increase in urea uptake into CFTR oocytes was significantly greater than for sham oocytes. I/F stimulated formate uptake into CFTR oocytes but not into sham oocytes. Fluxes of 22Na, 86Rb, 35SO4, 32PO4, and mannitol were unaltered by expression and activation of CFTR.

    本文言語English
    ページ(範囲)C1586-C1593
    ジャーナルAmerican Journal of Physiology - Cell Physiology
    266
    6 35-6
    DOI
    出版ステータスPublished - 1994

    ASJC Scopus subject areas

    • 生理学
    • 細胞生物学

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