Radially expanding transglial calcium waves in the intact cerebellum

Tycho M. Hoogland, Bernd Kuhn, Werner Göbel, Wenying Huang, Junichi Nakai, Fritjof Helmchen, Jane Flint, Samuel S.H. Wang

研究成果: Article査読

105 被引用数 (Scopus)


Multicellular glial calcium waves may locally regulate neural activity or brain energetics. Here, we report a diffusion-driven astrocytic signal in the normal, intact brain that spans many astrocytic processes in a confined volume without fully encompassing any one cell. By using 2-photon microscopy in rodent cerebellar cortex labeled with fluorescent indicator dyes or the calcium-sensor protein G-CaMP2, we discovered spontaneous calcium waves that filled approximately ellipsoidal domains of Bergmann glia processes. Waves spread in 3 dimensions at a speed of 4-11 μm/s to a diameter of ≈50 μm, slowed during expansion, and were reversibly blocked by P2 receptor antagonists. Consistent with the hypothesis that ATP acts as a diffusible trigger of calcium release waves, local ejection of ATP triggered P2 receptor-mediated waves that were refractory to repeated activation. Transglial waves represent a means for purinergic signals to act with local specificity to modulate activity or energetics in local neural circuits.

ジャーナルProceedings of the National Academy of Sciences of the United States of America
出版ステータスPublished - 2009 3 3

ASJC Scopus subject areas

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