Rad52 sumoylation and its involvement in the efficient induction of homologous recombination

Takashi Ohuchi, Masayuki Seki, Dana Branzei, Daisuke Maeda, Ayako Ui, Hideaki Ogiwara, Shusuke Tada, Takemi Enomoto

研究成果: Article

31 引用 (Scopus)

抜粋

The protein Rad52 is a key player in various types of homologous recombination and is essential to maintenance of genomic integrity. Although evidence indicates that Rad52 is modified by SUMO, the physiological relevance of this sumoylation remains unclear. Here, we identify the conditions under which Rad52 sumoylation is induced, and clarify the role of this modification in homologous recombination. Oligomerization of Rad52 was a prerequisite for sumoylation, and the modification occurred in the cell proceeding S phase being exposed to the DNA-damaging agent methyl methanesulfonate (MMS). Following exposure to MMS, sumoylated Rad52 accumulated in rad51 cells, but not in the recombination-related gene mutants, rad54, rad55, rad59, sgs1, or srs2. The accumulation of sumoylated Rad52 was suppressed in rad51 cells expressing Rad51-K191R, an ATPase-defective protein presumed to be recruited to ssDNA. Although the sumoylation defective mutant rad52-3KR (K10R/K11R/K220R) showed no defect in mating-type switching, which did not lead to Rad52 sumoylation in wild-type cells, the mutant did demonstrate a partial defect in MMS-induced interchromosomal homologous recombination.

元の言語English
ページ(範囲)879-889
ページ数11
ジャーナルDNA Repair
7
発行部数6
DOI
出版物ステータスPublished - 2008 6 1

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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    Ohuchi, T., Seki, M., Branzei, D., Maeda, D., Ui, A., Ogiwara, H., Tada, S., & Enomoto, T. (2008). Rad52 sumoylation and its involvement in the efficient induction of homologous recombination. DNA Repair, 7(6), 879-889. https://doi.org/10.1016/j.dnarep.2008.02.005