TY - JOUR
T1 - Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization
AU - Sato, M.
AU - Suzuki, S.
AU - Yasuda, Y.
AU - Kawauchi, H.
AU - Kanno, N.
AU - Sato, Y.
PY - 1988/11/1
Y1 - 1988/11/1
N2 - A new method for the quantitative analysis of acidic opines-alanopine, strombine, tauropine, and β-alanopine-is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines after partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, 10-30 pmol minimum detection, and better reproducibility than the high-voltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of β-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method.
AB - A new method for the quantitative analysis of acidic opines-alanopine, strombine, tauropine, and β-alanopine-is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines after partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, 10-30 pmol minimum detection, and better reproducibility than the high-voltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of β-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method.
KW - HPLC determination
KW - Mollusca
KW - imino acids
KW - metabolites
KW - opines
KW - phenylthiocarbamyl derivative
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U2 - 10.1016/0003-2697(88)90065-6
DO - 10.1016/0003-2697(88)90065-6
M3 - Article
C2 - 2467579
AN - SCOPUS:0024117196
SN - 0003-2697
VL - 174
SP - 623
EP - 627
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -