Quantitative HPLC analysis of acidic opines by phenylthiocarbamyl derivatization

M. Sato, S. Suzuki, Y. Yasuda, H. Kawauchi, N. Kanno, Y. Sato

研究成果: Article査読

22 被引用数 (Scopus)

抄録

A new method for the quantitative analysis of acidic opines-alanopine, strombine, tauropine, and β-alanopine-is presented. The method is based on formation of phenylthiocarbamyl (PTC) derivatives of the acidic opines after partial purification by ion-exchange chromatography. The PTC acidic opines are separated by reverse-phase high-performance liquid chromatography (HPLC) and detected within 20 min by ultraviolet absorbance. This HPLC method gives higher sensitivity, 10-30 pmol minimum detection, and better reproducibility than the high-voltage paper electrophoresis method. There is also good agreement for the three acidic opines (alanopine, strombine, and tauropine) when compared by HPLC and electrophoresis methods. Accumulation of β-alanopine was observed for the first time in the adductor muscle of blood shell, Scapharca broughtonii, during aerial exposure by application of the HPLC detection method.

本文言語English
ページ(範囲)623-627
ページ数5
ジャーナルAnalytical Biochemistry
174
2
DOI
出版ステータスPublished - 1988 11 1

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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