Quantification of molecules in1H-NMR metabolomics with formate as a concentration standard

Itiro Ando, Takuo Hirose, Tadashi Nemoto, Kazuhito Totsune, Yutaka Imai, Kazuhisa Takeuchi, Masako Fujiwara

研究成果: Article

10 引用 (Scopus)

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Quantitative analysis of metabolites is important in 1H-nuclear magnetic resonance (NMR)-based metabolomics of plasma. Human plasma contains a high density of proteins which heavily adsorb the commonly-used standard compound of sodium 3-(trimethylsilyl) propionate 2, 2, 3, 3-d4 (TSP). We have evaluated calcium formate as an alternative standard in 1D single-pulse 1H-NMR spectra to quantify plasma metabolites. Formate did not interact with either plasma metabolites or proteins under adequate conditions. Linear relations between the signal intensities and the added formate have been demonstrated in 1H spectra. The quantifications of glucose and creatinine by this method have shown good accordance with biochemical analysis. Calcium formate is applicable as a concentration standard to NMR metabolomics of plasma.

元の言語English
ページ(範囲)253-256
ページ数4
ジャーナルJournal of Toxicological Sciences
35
発行部数2
DOI
出版物ステータスPublished - 2010 5 5

ASJC Scopus subject areas

  • Toxicology

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