Pyruvate as a fluorescence quencher: A new spectroscopic assay for pyruvate reactions

Toshio Ando, Hidetake Miyata

研究成果: Article査読

12 被引用数 (Scopus)

抄録

Pyruvate ion, which is biologically ubiquitous and participates in many metabolic reactions, was found to be an effective quencher of fluorescence. Compared to other negatively charged quenchers such as I-, pyruvate is not toxic to proteins. By adding an inert, long-lived fluorophore to systems transacting pyruvate, it is possible to estimate activity by measuring the time course of the change in pyruvate quenching of the fluorophore. The procedure is illustrated by measuring the myosin subfragment-1 ATPase activity with a high sensitivity.

本文言語English
ページ(範囲)170-175
ページ数6
ジャーナルAnalytical Biochemistry
129
1
DOI
出版ステータスPublished - 1983 2 15
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

フィンガープリント

「Pyruvate as a fluorescence quencher: A new spectroscopic assay for pyruvate reactions」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル