TY - JOUR
T1 - Pyruvate as a fluorescence quencher
T2 - A new spectroscopic assay for pyruvate reactions
AU - Ando, Toshio
AU - Miyata, Hidetake
PY - 1983/2/15
Y1 - 1983/2/15
N2 - Pyruvate ion, which is biologically ubiquitous and participates in many metabolic reactions, was found to be an effective quencher of fluorescence. Compared to other negatively charged quenchers such as I-, pyruvate is not toxic to proteins. By adding an inert, long-lived fluorophore to systems transacting pyruvate, it is possible to estimate activity by measuring the time course of the change in pyruvate quenching of the fluorophore. The procedure is illustrated by measuring the myosin subfragment-1 ATPase activity with a high sensitivity.
AB - Pyruvate ion, which is biologically ubiquitous and participates in many metabolic reactions, was found to be an effective quencher of fluorescence. Compared to other negatively charged quenchers such as I-, pyruvate is not toxic to proteins. By adding an inert, long-lived fluorophore to systems transacting pyruvate, it is possible to estimate activity by measuring the time course of the change in pyruvate quenching of the fluorophore. The procedure is illustrated by measuring the myosin subfragment-1 ATPase activity with a high sensitivity.
KW - ATPase assay
KW - charged quencher
KW - fluorescence quenching
KW - pyruvate
KW - pyruvate reactions
UR - http://www.scopus.com/inward/record.url?scp=0021103390&partnerID=8YFLogxK
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U2 - 10.1016/0003-2697(83)90065-9
DO - 10.1016/0003-2697(83)90065-9
M3 - Article
C2 - 6222667
AN - SCOPUS:0021103390
SN - 0003-2697
VL - 129
SP - 170
EP - 175
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 1
ER -