Pyridoxine-selective fluorescence quenching by graphite oxide-containing molecularly imprinted polymers

A graphite oxide-containing molecularly imprinted polymer (GO-MIP) was prepared by using pyridoxine as a template molecule. The adsorption behavior of pyridoxine to GO-MIP was studied based on Scatchard analysis. The fluorescence of pyridoxine was quenched on the GO-MIP. In contrast, GO-MIP did not exhibit quenching to the fluorescence of nontemplated fluorescein. The fluorescence of pyridoxine was quenched more effectively by GO-MIP than by GO, confirming that the fluorescence was quenched through the selective binding of pyridoxine to the imprinted polymer.