Purification of ca2+/calmodulin-dependent protein kinase ii from rat stomach and its localization in the mucosa

Wei Jun Wang, Ko HJ1 Fukunaga, Satoshi Goto, Eishichi MIYAMoTo

研究成果: Article査読

1 被引用数 (Scopus)

抄録

Ca2+/calmodulin-dependent protein kinase II (CaM kinase II) with a Mr of 530,000 was purified from rat stomach. The enzyme showed a major protein band with 52 kDa and minor components with 50 and 54 kDa when examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The physicochemical and kinetic properties of the enzyme were similar to those of brain CaM kinase II. The enzyme showed a broad substrate specificity, and wascapable of phosphorylating several endogenous proteins in the cytosol and membrane fractions.The polyclonal antibody against brain CaM kinase II cross-reacted with the enzyme.Strong immunoreactivity was observed in the parietal cells of stomach by the immunohistochemical investigation. These results suggest that CaM kinase II regulates the Ca2+-dependent cellular functions of the parietal cells.

本文言語English
ページ(範囲)231-240
ページ数10
ジャーナルBiomedical Research (Japan)
15
4
DOI
出版ステータスPublished - 1994

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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