Purification, crystallization and preliminary X-ray diffraction analysis of the Kelch-like motif region of mouse Keap1

Balasundaram Padmanabhan, Maria Scharlock, Kit I. Tong, Yoshihiro Nakamura, Moon Il Kang, Akira Kobayashi, Takehisa Matsumoto, Akiko Tanaka, Masayuki Yamamoto, Shigeyuki Yokoyama

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Keap1 (Kelch-like ECH-associating protein 1) is a negative regulator of the Nrf2 transcription factor in the cytoplasm. The Kelch/DGR (double-glycine repeat) domain of Keap1 associates with Nrf2 as well as with actin filaments. A recombinant protein containing both the Kelch/DGR domain and the C-terminal region of mouse Keap1 was expressed in Escherichia coli, purified to near-homogeneity and crystallized by the sitting-drop vapour-diffusion method. The crystal belongs to space group P61 or P65, with unit-cell parameters a = b = 102.95, c = 55.21 Å, and contains one molecule in the asymmetric unit. A complete diffraction data was collected to 2.25 Å resolution using an R-AXIS IV++ imaging plate mounted on an RA-Micro7 Cu 14;Kα rotating-anode X-ray generator.

本文言語English
ページ(範囲)153-155
ページ数3
ジャーナルActa Crystallographica Section F: Structural Biology and Crystallization Communications
61
1
DOI
出版ステータスPublished - 2005
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 遺伝学
  • 凝縮系物理学

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