Purification and some properties of two anionic trypsins from the eel (anguilla japonica)

Reiji Yoshinaka, Mamoru Sato, Tohru Suzuki, Shizunori Ikeda

研究成果: Article査読

18 被引用数 (Scopus)

抄録

1. 1. Two anionic enzymes, designated as trypsins 1 and 2, were purified from the pancreas of the eel Anguilla japonica by DEAE-cellulose column chromatography and Sephadex G-75 gel filtration. 2. 2. The final preparation of trypsin 1 was homogeneous but that of trypsin 2 still contained impurities. 3. 3. Both enzymes had similar pH optima of near 8.3 for the hydrolysis of N-tosyl-l-arginine methyl ester. 4. 4. Trypsin 1 was stabilized by calcium ions but the stability of trypsin 2 was not affected by calcium ions. 5. 5. Both enzymes were inhibited by typical trypsin inhibitors including serine proteinase inhibitors.

本文言語English
ページ(範囲)5-9
ページ数5
ジャーナルComparative Biochemistry and Physiology -- Part B: Biochemistry and
80
1
DOI
出版ステータスPublished - 1985
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 分子生物学

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