Purification and characterization of an antibacterial and antineoplastic protein secretion of a sea hare, Aplysia juliana

Hisao Kamiya, Koji Muramoto, Rina Goto, Masahiro Sakai, Yoshio Endo, Masatoshi Yamazaki

    研究成果: Article査読

    35 被引用数 (Scopus)

    抄録

    H. Kamiya, K. Muramoto, R. Goto, M. Sakai, Y. Endo and M. Yamazaki. Purification and characterization of an antibacterial and antineoplastic protein secretion of a sea hare, Aplysia juliana. Toxicon 27, 1269-1277, 1989. The fetid secretion of a sea hare, Aplysia juliana, was lethal to crabs and also inhibited the growth of bacteria. When the secretion was partitioned between water and n-hexane, only the n-hexane layer, which had a nauseating odor, was lethal to crabs. The water-soluble fraction showed strong antibacterial activity and inhibited the growth of both Gram-positive and Gram-negative bacteria. Antibacterial activity of the water-soluble fraction was destroyed by heating at 50°C for 15 min, but was resistant to treatment with proteolytic enzymes. The active principle, named julianin-S, was purified by gel filtration and ion exchange chromatography. The purified specimen gave a single protein showing a mol. wt of approximately 67,000, as determined by gel filtration. Julianin-S inhibited the growth of Bacillus subtilis by 50% at a concentration of 70 ng protein/ml. It was also cytotoxic to murine tumor cells and inhibited in vitro growth of L1210 cells by 50% at a concentration of 8 ng protein/ml.

    本文言語English
    ページ(範囲)1269-1277
    ページ数9
    ジャーナルToxicon
    27
    12
    DOI
    出版ステータスPublished - 1989

    ASJC Scopus subject areas

    • 毒物学

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