Purification and Characterization of a Ca2+‐ and Calmodulin‐Dependent Protein Kinase from Rat Brain

Kohji Fukunaga, Hideyuki Yamamoto, Kazuo Matsui, Kenji Higashi, Eishichi Miyamoto

研究成果: Article査読

148 被引用数 (Scopus)

抄録

Abstract: A Ca2+‐ and calmodulin‐dependent protein kinase was purified from rat brain cytosol fraction to apparent homogeneity at approximately 800‐fold and with a 5% yield. The purified enzyme had a molecular weight of 640,000 as determined by gel filtration analysis on Sephacryl S‐300 and a sedimentation coefficient of 15.3 S by sucrose density gradient centrifugation, and resulted in a single protein band of MW 49,000 by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis. These results suggest that the native enzyme has a large molecular weight and consists of 11 to 14 identical subunits. The purified enzyme exhibited Km values of 109 and 30 μM for ATP and chicken gizzard myosin light chain, respectively, and Ka values of 12 nM and 1.9 μM for brain calmodulin and Ca2+, respectively. In addition to myosin light chain, myelin basic protein, casein, arginine‐rich histone, microtubule protein, and synaptosomal proteins were phosphorylated by the enzyme in a Ca2+‐ and calmodulin‐dependent manner. The purified enzyme was phosphorylated without the addition of the catalytic subunit of cyclic AMP‐dependent protein kinase. Our findings indicate that there is a multifunctional Ca2+‐ and calmodulin‐dependent protein kinase in the brain and that this enzyme may regulate the reactions of various endogenous proteins.

本文言語English
ページ(範囲)1607-1617
ページ数11
ジャーナルJournal of Neurochemistry
39
6
DOI
出版ステータスPublished - 1982 12月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 細胞および分子神経科学

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