TY - JOUR
T1 - Pulsed EPR Analysis of the Photo-Induced Triplet Radical Pair in the BLUF Protein SyPixD
T2 - Determination of the Protein-Protein Distance and Orientation in the Oligomeric Protein
AU - Kondo, Toru
AU - Masuda, Shinji
AU - Mino, Hiroyuki
N1 - Funding Information:
Acknowledgments This work was supported by grant-in-aid for scientific research KAKENHI (21370069) to H.M., a Japan Society for the Promotion of Science (JSPS) research fellowship for young scientists (nr. 21008983) to T.K., and Japan Science and Technology Agency program of Precursory Research for Embryonic Science and Technology (JST PRESTO) to S.M.
PY - 2011/8
Y1 - 2011/8
N2 - A photo-induced radical pair of FADH· and Y8· and in BLUF protein SyPixD was studied by pulsed electron paramagnetic resonance (EPR) spectroscopy. Blue light illumination at 150 K for 30 min followed by cooling to 50 K during illumination induced the stable radical pair. The EPR signal has been characterized by a Pake doublet signal with complete S = 1 spin state. The radical pair was utilized as a probe to analyze the oligomer of SyPixD. The relative arrangement of PixD proteins in the complex was investigated by pulsed electron-electron double resonance (PELDOR) with the orientation selection. Based on the decameric structure in the crystal, the possible structure for the PELDOR results was discussed.
AB - A photo-induced radical pair of FADH· and Y8· and in BLUF protein SyPixD was studied by pulsed electron paramagnetic resonance (EPR) spectroscopy. Blue light illumination at 150 K for 30 min followed by cooling to 50 K during illumination induced the stable radical pair. The EPR signal has been characterized by a Pake doublet signal with complete S = 1 spin state. The radical pair was utilized as a probe to analyze the oligomer of SyPixD. The relative arrangement of PixD proteins in the complex was investigated by pulsed electron-electron double resonance (PELDOR) with the orientation selection. Based on the decameric structure in the crystal, the possible structure for the PELDOR results was discussed.
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U2 - 10.1007/s00723-011-0237-1
DO - 10.1007/s00723-011-0237-1
M3 - Article
AN - SCOPUS:79960729652
VL - 40
SP - 545
EP - 555
JO - Applied Magnetic Resonance
JF - Applied Magnetic Resonance
SN - 0937-9347
IS - 4
ER -