Pull-down method to access the cell surface receptor for Toxoplasma gondii

Haiyan Gong, Kyousuke Kobayashi, Tatsuki Sugi, Hitoshi Takemae, Taisuke Horimoto, Xuenan Xuan, Hiroomi Akashi, Kentaro Kato

研究成果: Article査読

抄録

Protein X, which is expressed on the surface of Toxoplasma and is thought to interact with host molecules, was expressed as a GST recombinant protein, conjugated to sepharose 4B, and used to pull down biotin-labeled 293T cells. The product was analysis by 2D-PAGE and Western blotting. Mass spectrometry revealed the reacted spots from the gel to be heat shock proteins.

本文言語English
ページ(範囲)514-515
ページ数2
ジャーナルParasitology International
65
5
DOI
出版ステータスPublished - 2016 10 1
外部発表はい

ASJC Scopus subject areas

  • 寄生虫科
  • 感染症

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