Proteomic analysis of early melanosomes: Identification of novel melanosomal proteins

Venkatesha Basrur, Feng Yang, Tsuneto Kushimoto, Youichiro Higashimoto, Ken ichi Yasumoto, Julio Valencia, Jacqueline Muller, Wilfred D. Vieira, Hidenori Watabe, Jeffrey Shabanowitz, Vincent J. Hearing, Donald F. Hunt, Ettore Appella

研究成果: Article査読

122 被引用数 (Scopus)

抄録

Melanin is a heterogeneous biopolymer produced only by specific cells termed melanocytes, which synthesize and deposit the pigment in specialized membrane-bound organelles known as melanosomes. Although melanosomes have been suspected of being closely related to lysosomes and platelets, the total number of melanosomal proteins is still unknown. Thus far, six melanosome-specific proteins have been identified, and the challenge is to characterize the complete proteome of the melanosome to further understand its mechanism of biogenesis. In this report, we used mass spectrometry and subcellular fractionation to identify protein components of early melanosomes. Using this approach, we have identified all 6 of the known melanosome-specific proteins, 56 proteins that are shared with other organelles, and confirmed the presence of 6 novel melanosomal proteins using western blotting and by immunohistochemistry.

本文言語English
ページ(範囲)69-79
ページ数11
ジャーナルJournal of Proteome Research
2
1
DOI
出版ステータスPublished - 2003
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 化学 (全般)

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