Protein folding dynamics detected by time-resolved synchrotron X-ray small-angle scattering technique

Tetsuro Fujisawa, Satoshi Takahashi

研究成果: Conference contribution

抄録

The polypeptide collapse is an essential dynamics in protein folding. To understand the mechanism of the collapse, in situ observation of folding by various probes is necessary. The changes in secondary and tertiary structures in the folding process of globular proteins, whose chain lengths are less than 300 polypeptides, were observed by circular dichrosim and intrinsic fluorescence spectroscopies, respectively. On the other hand, those in protein compactness could be only detected by using time-resolved synchrotron x-ray small-angle scattering technique. The observed dynamics for several proteins with different topologies suggested a common folding mechanism termed "collapse and search" dynamics, in which the polypeptide collapse precedes the formation of the native contact formation. In "collapse and search" dynamics, the most outstanding feature lied in the compactness of the initial intermediates. The collapsed intermediates demonstrated the scaling relationship between radius of gyration (R g ) and chain length with a scaling exponent of 0.35 ± 0.11, which is close to the value (1/3) predicted by mechano-statistical theory for the collapsed globules of polymers in poor solvent. Thus, it was suggested that the initial collapse is caused by the coil-globule transition of polymers. Since the collapse is essential to the folding of larger proteins, further investigations on the collapse likely lead to an important insight into the protein folding phenomena.

本文言語English
ホスト出版物のタイトルPORTABLE SYNCHROTRON LIGHT SOURCES AND ADVANCED APPLICATIONS
ホスト出版物のサブタイトル2nd International Symposium on Portable Synchrotron Light Sources and Advanced Applications
ページ89-92
ページ数4
DOI
出版ステータスPublished - 2007 5 11
外部発表はい
イベント2nd International Symposium on Portable Synchrotron Light Sources and Advanced Applications - Shiga, Japan
継続期間: 2007 1 152007 1 17

出版物シリーズ

名前AIP Conference Proceedings
902
ISSN(印刷版)0094-243X
ISSN(電子版)1551-7616

Conference

Conference2nd International Symposium on Portable Synchrotron Light Sources and Advanced Applications
国/地域Japan
CityShiga
Period07/1/1507/1/17

ASJC Scopus subject areas

  • 物理学および天文学(全般)

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