Protein C (OprC) of the outer membrane of Pseudomonas aeruginosa is a copper-regulated channel protein

Hiroshi Yoneyama, Taiji Nakae

研究成果: Article査読

50 被引用数 (Scopus)

抄録

Protein C (OprC) of the outer membrane of Pseudomonas aeruginosa forms small channels, as assayed by the liposome swelling method. We report here that OprC functions as a channel-forming and copper-binding protein. OprC purified to homogeneity formed a channel in planar lipid bilayers with an ion conductance of about 200 pS in 1 M NaCl. Cloning and sequencing of the gene encoding OprC revealed that it specified a polypeptide comprising 723 and 668 amino acid residues for the precursor and mature polypeptides (M(r) 73,372), respectively. The amino acid sequence of OprC showed the highest degree of similarity with that of NosA of Pseudomonas stutzeri (65% sequence identity) which conveys Cu2+ to intracellular acceptor(s). OprC showed high copper-binding activity (K(d) = 2.6 μM) in aqueous solution containing surfactant. The expression of OprC appeared to be repressed by exogenous Cu2+ and derepressed by anaerobiosis in the presence of nitrate. These results suggest that OprC might be involved in copper utilization.

本文言語English
ページ(範囲)2137-2144
ページ数8
ジャーナルMicrobiology
142
8
DOI
出版ステータスPublished - 1996 8月
外部発表はい

ASJC Scopus subject areas

  • 微生物学

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