Protein antiadsorption modification by negatively charged phospholipid polymer for microsystem needing electroosmotic flow

Yan Xu, Madoka Takai, Tomohiro Konno, Kazuhiko Ishihara

研究成果: Paper査読

抄録

Biointerface with cell-membrane-like structure was constructed on the quartz microfluidic chip to suppress the non-specific protein adsorption as well as to retain the electroosomotic flow (EOF) effect through one-step modification. The PMBSSi, a negatively charged phospholipid copolymer containing 2-methacryloyloxyethyl phosphorylcholine (MPC) units, was synthesized to introduce such cell-membrane-like segments as well as surface charges onto the silica based microchannels via chemical bonding of the silane coupling units in the copolymer with the substrates. At neutral pH, the interface constructed by PMBSSi modification retained a significant cathodal EOF. Although the PMBSSi possesses negative charges, the nonspecific adsorptions of several kinds of proteins were effectively suppressed.

本文言語English
ページ215-217
ページ数3
出版ステータスPublished - 2006 1 1
外部発表はい
イベント10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan
継続期間: 2006 11 52006 11 9

Other

Other10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006
国/地域Japan
CityTokyo
Period06/11/506/11/9

ASJC Scopus subject areas

  • 化学工学(その他)
  • バイオエンジニアリング

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