Protective effect of lycopene on oxidative stress-induced cell death of pancreatic acinar cells

Jeong Yeon Seo, Atsushi Masamune, Tooru Shimosegawa, Hyeyoung Kim

研究成果: Conference contribution

26 被引用数 (Scopus)

抄録

Previously we showed that the underlying mechanism of oxidative stress-induced apoptosis is nuclear loss of DNA repair protein Ku70 and Ku80, which are involved in the DNA repair process of double-strand breaks. Lycopene acts as an antioxidant and a singlet oxygen quencher. In the present study, we aim to investigate whether lycopene protects oxidative stress-induced cell death of pancreatic acinar AR42J cells by preventing the loss of Ku70 in the nucleus. The cells received oxidative stress caused by glucose oxidase acting on β-D-glucose (glucose/glucose oxidase) and were cultured in the absence or presence of various concentrations of lycopene. Viable cell numbers, the levels of H2O2 in the medium, level of Ku70 protein, and Ku-DNA-binding activity were determined. As a result, glucose/glucose oxidase induced the decrease in cell viability, increase in H2O2 production, decrease in Ku70 levels in whole-cell extracts and nuclear extracts, and decrease in Ku-DNA-binding activity of AR42J cells. Lycopene inhibited glucose/glucose oxidase-induced cell death by preventing nuclear loss of Ku70 and a decrease in Ku-DNA-binding activity of AR42J cells. In conclusion, lycopene may be beneficial for the treatment of oxidative stress-induced cell death by preventing loss of DNA repair protein Ku70.

本文言語English
ホスト出版物のタイトルNatural Compounds and Their Role in Apoptotic Cell Signaling Pathways
出版社Blackwell Publishing Inc.
ページ570-575
ページ数6
ISBN(印刷版)9781573317375
DOI
出版ステータスPublished - 2009 8

出版物シリーズ

名前Annals of the New York Academy of Sciences
1171
ISSN(印刷版)0077-8923
ISSN(電子版)1749-6632

ASJC Scopus subject areas

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 科学史および科学哲学

フィンガープリント

「Protective effect of lycopene on oxidative stress-induced cell death of pancreatic acinar cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル