Proposal of new modification technique for linear double-stranded DNAs using the polysaccharide schizopyllan

Takahisa Anada, Hideshi Matsunaga, Ryouji Karinaga, Kazuya Koumoto, Masami Mizu, Koji Nakano, Seiji Shinkai, Kazuo Sakurai

研究成果: Article査読

8 被引用数 (Scopus)

抄録

A poly(dA) tailed double-stranded DNA and its polysaccharide complexes are reported. A natural polysaccharide schizophyllan (SPG) has been known to form a stable complex with poly(dA). We attached a poly(dA) 80 tail to the both ends of a linear double-stranded DNA, which had been prepared from a plasmid DNA vector. The poly(dA) tailed DNA verified to form complex with SPG by gel electrophoresis and atomic force microscopy (AFM). AFM images indicated that the complexes exhibit a dumbbell-like architecture, that is, quite similar to that of adenovirus genome. The complex demonstrated excellent exonuclease resistance, probably because of the protection effect by SPG complexation.

本文言語English
ページ(範囲)5655-5659
ページ数5
ジャーナルBioorganic and Medicinal Chemistry Letters
14
22
DOI
出版ステータスPublished - 2004 11 15

ASJC Scopus subject areas

  • 生化学
  • 分子医療
  • 分子生物学
  • 薬科学
  • 創薬
  • 臨床生化学
  • 有機化学

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