Properties of two VEGF receptors, Flt-1 and KDR, in signal transduction

Yasufumi Sato, Shinichi Kanno, Nobuyuki Oda, Mayumi Abe, Mikito Ito, Kenya Shitara, Masabumi Shibuya

研究成果: Article査読

118 被引用数 (Scopus)

抄録

The properties of two VEGF receptors, Flt-1 and KDR, in the signal transduction of VEGF in human umbilical vein endothelial cells (HUVECs) were investigated by using two newly developed blocking monoclonal antibodies (mAbs) against Flt-1 and KDR. VEGF stimulated the expression of transcription factor Ets-1 as well as matrix metalloproteinase-1 (MMP-1) and Flt-1 in HUVECs. The KDR/Flt-1 heterodimer and the KDR homodimer mediate the expression of Ets-1, MMP-1, and Flt-1. VEGF also stimulated DNA synthesis and migration of HUVECs. DNA synthesis is mediated by the same signaling system as the expression of Ets-1. In contrast, cell migration is regulated by two distinct signaling systems. The Flt-1 homodimer is required for actin reorganization. The KDR/Flt-1 heterodimer and the KDR homodimer are required for the assembly of vinculin in focal adhesion plaque by regulating the phosphorylation of focal adhesion kinase (FAK) and paxillin.

本文言語English
ページ(範囲)201-207
ページ数7
ジャーナルAnnals of the New York Academy of Sciences
902
DOI
出版ステータスPublished - 2000

ASJC Scopus subject areas

  • 神経科学(全般)
  • 生化学、遺伝学、分子生物学(全般)
  • 科学史および科学哲学

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