A 100 000 g pellet fraction of a strain of Botryococcus braunii B race had the capability of phosphorylating farnesol in the presence of CTP to give its mono- and diphosphate esters, but the same fraction did not phosphorylate other isoprenoid alcohols, including geraniol, geranylgeraniol, Z,E-polyprenols and dolichols. Exogenously added farnesyl monophosphate was not phosphorylated by this fraction. The Km values in the formation of farnesyl monophosphate were 0.5 μM and 0.2 mM for farnesol and CTP, respectively. Iodoacetamide strongly inhibited the formation of farnesyl diphosphate, but had no effect on that of farnesyl monophosphate.
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