Prolyl isomerase Pin1 promotes proplatelet formation of megakaryocytes via tau

Taiki Shimizu, Chiyoko Uchida, Ritsuko Shimizu, Hozumi Motohashi, Takafumi Uchida

研究成果: Article査読

8 被引用数 (Scopus)

抄録

Here we show that Pin1, a peptidyl-prolyl cis/trans isomerase which catalyzes the isomerization of phosphorylated Ser/Thr-Pro, is a regulatory molecule of thrombopoiesis. We found that mice lacking the Pin1 gene (Pin1−⁄− mice) formed more megakaryocytes (MKs) than wild type mice (WT mice), and that the proplatelet formation of MKs was poorer in Pin1−⁄− mice than WT mice. Treatment of Meg-01 cells, a megakaryoblastic floating cell line, with shRNA against Pin1 suppressed the proplatelet formation. Expression of tau, a microtubule associated protein was induced in MKs during proplatelet formation. Pin1 bound tau and promoted microtubule polymerization. Our results show that Pin1 serves as a positive regulatory molecule of proplatelet formation of MKs by enhancing the function of phosphorylated tau.

本文言語English
ページ(範囲)946-951
ページ数6
ジャーナルBiochemical and biophysical research communications
493
2
DOI
出版ステータスPublished - 2017 11月 18

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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