Production of phleichrome by Cladosporium phlei as stimulated by diketopiperadines of Epichloe typhina

Yoshiya Seto, Yasunori Kogami, Tadayuki Shimanuki, Kosaku Takahashi, Hideyuki Matsuura, Teruhiko Yoshihara

    研究成果: Article査読

    17 被引用数 (Scopus)

    抄録

    Epichloe typhina is an endophytic fungus, while Cladosporium phlei is a pathogenic fungus of the timothy plant (Phleum pretense L.). We found two activities in the culture filtrate of E. typhina: one stimulated the pathogenic fungus, C. phlei, to produce phleichrome and the other inhibited its growth. The active ingredients that stimulated the production of phleichrome and inhibited the growth of C. phlei were isolated and characterized. The isolated compounds were identified as cyclo-(L-Pro-L-Leu) and cyclo-(L-Pro-L-Phe), which were stimulatory compounds, and p-hydroxybenzaldehyde, which was the growth inhibitory compound, based on an analysis of their spectral data. Of the two stimulatory compounds, cycto-(L-Pro-L-Phe) showed higher activity. However, when 500 μg of cyclo-(L-Pro-L-Phe) was spotted on the TLC plate for bio-autography, a growth inhibitory zone was identified in the central red region, which contained phleichrome. On the other hand, phleichrome showed antifungal activity against E. typhina in the light, so it is assumed that there might be antagonism between the endophytic fungus, E. typhina, and the pathogenic fungus, C. phlei.

    本文言語English
    ページ(範囲)1515-1519
    ページ数5
    ジャーナルBioscience, Biotechnology and Biochemistry
    69
    8
    DOI
    出版ステータスPublished - 2005 8月 23

    ASJC Scopus subject areas

    • バイオテクノロジー
    • 分析化学
    • 生化学
    • 応用微生物学とバイオテクノロジー
    • 分子生物学
    • 有機化学

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