Production of an immunoglobulin gene product by the plasmid expression vector L factor in mouse myeloma cells

Hiroshi Ishikawa, Katsuhiko Nishimori, Takashi Kohda, Hiroshi Saito, Michio Oishi

研究成果: Article査読

抄録

L factor, originally discovered in a subclone of mouse L cells, is a multicopy mammalian plasmid whose structure is related to that of polyoma. When a composite DNA consisting of L factor, pBR, bacterial neo, and an immunoglobulin (kappa) gene was introduced into mouse myeloma cells, the DNA was established as plasmids in the cells without rearrangement or integration into the chromosomes. The plasmid-bearing myeloma cells produced kappa mRNA and the gene product, kappa immunoglobulin, which were apparently derived from the gene located on plasmid L factor. These results suggest that L factor can be used as a plasmid expression vector for studies on gene expression and production of biologically active substances in mammalian cells.

本文言語English
ページ(範囲)93-100
ページ数8
ジャーナルPlasmid
28
2
DOI
出版ステータスPublished - 1992 9

ASJC Scopus subject areas

  • 分子生物学

フィンガープリント

「Production of an immunoglobulin gene product by the plasmid expression vector L factor in mouse myeloma cells」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル