Production and secretion of adrenomedullin in cultured human alveolar macrophages

Masaharu Nakayama; Kazuhiro Takahashi; Osamu Murakami; Masaru Yanai; Hidetada Sasaki; Kunio Shirato; Shigeki Shibahara

doi:10.1016/S0196-9781(99)00107-2

Production and secretion of adrenomedullin in cultured human alveolar macrophages

Masaharu Nakayama, Kazuhiro Takahashi, Osamu Murakami, Masaru Yanai, Hidetada Sasaki, Kunio Shirato, Shigeki Shibahara

研究成果: Article › 査読

18 被引用数 (Scopus)

抄録

To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/10⁵ cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

本文言語	English
ページ（範囲）	1123-1125
ページ数	3
ジャーナル	Peptides
巻	20
号	9
DOI	https://doi.org/10.1016/S0196-9781(99)00107-2
出版ステータス	Published - 1999 9月

ASJC Scopus subject areas

生化学
生理学
内分泌学
細胞および分子神経科学

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10.1016/S0196-9781(99)00107-2

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title = "Production and secretion of adrenomedullin in cultured human alveolar macrophages",

abstract = "To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.",

keywords = "Adrenomedullin, Alveolar macrophages",

author = "Masaharu Nakayama and Kazuhiro Takahashi and Osamu Murakami and Masaru Yanai and Hidetada Sasaki and Kunio Shirato and Shigeki Shibahara",

note = "Funding Information: This work has been supported partly by Grants-in-aid for Scientific Research (B and C) and for Exploratory Research from the Ministry of Education, Science, Sports and Culture of Japan, and by the Gonryou Medical Foundation.",

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AU - Nakayama, Masaharu

AU - Takahashi, Kazuhiro

AU - Murakami, Osamu

AU - Yanai, Masaru

AU - Sasaki, Hidetada

AU - Shirato, Kunio

AU - Shibahara, Shigeki

N1 - Funding Information: This work has been supported partly by Grants-in-aid for Scientific Research (B and C) and for Exploratory Research from the Ministry of Education, Science, Sports and Culture of Japan, and by the Gonryou Medical Foundation.

PY - 1999/9

Y1 - 1999/9

N2 - To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

AB - To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

KW - Adrenomedullin

KW - Alveolar macrophages

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Production and secretion of adrenomedullin in cultured human alveolar macrophages

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