Production and secretion of adrenomedullin in cultured human alveolar macrophages

Masaharu Nakayama, Kazuhiro Takahashi, Osamu Murakami, Masaru Yanai, Hidetada Sasaki, Kunio Shirato, Shigeki Shibahara

研究成果: Article査読

18 被引用数 (Scopus)

抄録

To explore the role of adrenomedullin (ADM) in macrophages, we investigated the secretion of ADM by alveolar macrophages. Human alveolar macrophages obtained from bronchoalveolar lavage were cultured for 24 h. Northern blot analysis revealed ADM mRNA expression in alveolar macrophages. The levels of immunoreactive ADM in the media were 0.89 ± 0.12 fmol/105 cells/24 h (n = 10). Reverse-phase high-performance liquid chromatography of the extract of culture media showed one major peak eluting in the position of the human ADM standard. The present study shows that alveolar macrophages produce and secrete ADM. Copyright (C) 1999 Elsevier Science Inc.

本文言語English
ページ(範囲)1123-1125
ページ数3
ジャーナルPeptides
20
9
DOI
出版ステータスPublished - 1999 9月

ASJC Scopus subject areas

  • 生化学
  • 生理学
  • 内分泌学
  • 細胞および分子神経科学

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