Production and secretion in Escherichia coli of hepatitis b virus pre-S2 antigen as fusion proteins with β-lactamase

H. Kadokura, K. Yoda, M. Imai, M. Yamasaki

研究成果: Article査読

4 被引用数 (Scopus)

抄録

The diagnostically important surface antigen pre-S2 of hepatitis B virus was produced in large amounts in the periplasmic space of Escherichia coli. The DNA fragments (pre-S2) coding the pre-S2 antigen were tandemly duplicated or triplicated and ligated in the same reading frame to a fragment containing the promoter and the signal sequence of the alkaline phosphatase-coding gene (phoA) of E. coli. Further, a DNA fragment (bla) coding mature β-lactamase was joined to the region coding the C terminus of the pre-S2 repeat to stabilize the gene product. Upon induction of the phoA-(pre-S2)3-bla fusion gene, the fusion protein was produced at up to 30% of the total cellular protein. Fractionation of the cellular components and trypsin accessibility of the product showed that the antigen was secreted in the periplasm and formed inclusion bodies there. The signal sequence of alkaline phosphatase was found to be correctly processed in E. coli.

本文言語English
ページ(範囲)2742-2747
ページ数6
ジャーナルApplied and environmental microbiology
56
9
DOI
出版ステータスPublished - 1990
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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