Probing intra-molecular mechanics of single circularly permuted green fluorescent protein with atomic force microscopy

Tong Wang, Ken Nakajima, Atsushi Miyawaki, Masahiko Hara

    研究成果: Article査読

    6 被引用数 (Scopus)

    抄録

    We investigated the mechanical unfolding of single circularly permuted green fluorescent protein (cpGFP) with atomic force microscopy (AFM). The molecule was stretched from its N- and C-termini by an external force causing an elongation of the polypeptide chain up to its full length. The features of the force-extension (F-E) curves were found to depend on the stretching speed. At fast speeds, we detected one peak in the F-E curves before final rupture of the extended molecule, which we interpreted as the unfolding of two terminal halves within cpGFP. We observed several more force peaks in a sawtooth pattern at much slower speeds, and explained the appearance of such force peaks as cooperative unfolding of the hidden sub-structures inside each terminal half.

    本文言語English
    ページ(範囲)90-95
    ページ数6
    ジャーナルUltramicroscopy
    105
    1-4
    DOI
    出版ステータスPublished - 2005 11

    ASJC Scopus subject areas

    • 電子材料、光学材料、および磁性材料
    • 原子分子物理学および光学
    • 器械工学

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