Primary structure of sheep prostaglandin endoperoxide synthase deduced from cDNA sequence

Chieko Yokoyama, Toshiyuki Takai, Tadashi Tanabe

研究成果: Article査読

172 被引用数 (Scopus)

抄録

The complete amino acid sequence of prostaglandin endoperoxide synthase from sheep vesicular gland has been deduced by cloning and sequence analysis of DNA complementary to its messenger RNA. The results were confirmed by digestion of the enzyme with carboxypeptidase Y and by automated Edman degradation of the intact enzyme polypeptide and peptide fragments obtained by limited proteolysis of the enzyme with Achromobacter proteinase I. Mature sheep prostaglandin endoperoxide synthase is shown to be composed of 576 amino acids with an Mr of 66 175. The precursor peptide is predicted to contain a 24-residue signal peptide. The serine residue susceptible to acetylation by aspirin is found to be located near the C-terminus of the enzyme polypeptide.

本文言語English
ページ(範囲)347-351
ページ数5
ジャーナルFEBS Letters
231
2
DOI
出版ステータスPublished - 1988 4 25
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 構造生物学
  • 生化学
  • 分子生物学
  • 遺伝学
  • 細胞生物学

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