Primary structure and functional expression from cDN A of the cardiac ryanodine receptor/calcium release channel

Junichi Nakai, Toshiaki Imagawa, Yasuhiro Hakamata, Munekazu Shigekawa, Hiroshi Takeshima, Shosaku Numa

研究成果: Article査読

277 被引用数 (Scopus)

抄録

The sequence of 4968 (or 4976 with an insertion) amino acids composing the ryanodine receptor from rabbit cardiac sarcoplasmic reticulum has been deduced by cloning and sequencing the cDNA. This protein is homologous in amino acid sequence and shares characteristic structural features with the skeletal muscle ryanodine receptor. Xenopus oocytes injected with mRNA derived from the cardiac ryanodine receptor cDNA exhibit Ca2+-dependent Cl- current in response to caffeine, which indicates the formation of functional calcium release channels. RNA blot hybridization analysis with a probe specific for the cardiac ryanodine receptor mRNA shows that the stomach and brain contain a hybridizable RNA species with a size similar to that of the cardiac mRNA. This result, in conjunction with cloning and analysis of partial cDNA sequences, suggests that the brain contains a cardiac type of ryanodine receptor mRNA.

本文言語English
ページ(範囲)169-177
ページ数9
ジャーナルFEBS Letters
271
1-2
DOI
出版ステータスPublished - 1990 10 1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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