Presence of protein-bound unconjugated bile acids in the cytoplasmic fraction of rat brain

Nariyasu Mano, Takaaki Goto, Masashi Uchida, Koji Nishimura, Masayuki Ando, Norihiro Kobayashi, Junichi Goto

研究成果: Article査読

86 被引用数 (Scopus)

抄録

Using liquid chromatography/electrospray ionization mass spectrometry, we have found three unconjugated bile acids [cholic acid (CA), chenodeoxycholic acid (CDCA), and deoxycholic acid (DCA)] in the rat brain cytoplasmic fraction. CDCA was detected only upon extraction with high concentrations of guanidine, indicating that it is bound noncovalently to protein in the brain. The most abundant of the three, it was present at a concentration of 1.6 nmol/g wet weight (∼15 mg of protein) of brain, corresponding to almost 30 times its serum concentration. CA and DCA were present at 1/30th the concentration of CDCA. Bile acids conjugated with amino acids, sulfuric acid, and glucuronic acid were not detected. These data clearly demonstrate that unconjugated CDCA and, to a lesser extent, CA and DCA, exists in the rat brain.

本文言語English
ページ(範囲)295-300
ページ数6
ジャーナルJournal of lipid research
45
2
DOI
出版ステータスPublished - 2004 2月

ASJC Scopus subject areas

  • 生化学
  • 内分泌学
  • 細胞生物学

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