Preparation of cyto-compatible phospholipid polymer matrix formed by self-closslinkage

We propose a novel polymeric hydrogel through a covalent complex formation between two kinds of water-soluble polymers. In this study, a 2-methacryloyloxyehtyl phosphorlcholine (MPC) polymer (PMBV) was copolymerized with an n-butyl methacrylate and a vinyl phenylboronic acid by a conventional radical polymerization. The PMBV containing a phenylboronic acid could form a spontaneous hydrogel formation between the polyol (diol) compounds such as poly (vinyl alcohol)(PVA). The hydrogel was formed in the water and the biological fluid, and dissociated by respond to biological components such as glucose. In this study, we report the synthesis of novel MPC polymer containing phenylboronic acid, and preparation of the hydrogel with P VA. The basic chatracterization of formed PMBV hydrogel was evaluated, and the hydrogel was used as 3D matrix to culture the cells. Mouse fibroblast cell line (L929) was cultured in the PMBV hydrogel. Although, the cells were suspended and permeated in the hydrogel, they did not aggregate together. After dissociation of the hydrogel, the cells which were cultured in the matrix were seeded on the conventional tissue culture polystyrene. The cells could adhere and proliferate on the surface. We concluded that the PMBV hydrogel could be applied as 3D cell culture matrix.