Preparation of an organized film composed of polymers, avidin, and concanavalin A and its binding properties

Junichi Anzai, Sumiko Akase

研究成果: Article査読

5 被引用数 (Scopus)

抄録

The multilayer thin films composed of polymers, avidin, and concanavalin A (Con A) were prepared on the surface of a quartz slide by alternate deposition of anionic and cationic polymers and the proteins. The loading of avidin and Con A in the film was evaluated spectroscopically by using Texas Red tagged avidin and Con A. Avidin molecules assembled in the film caused its binding activity toward biotin and analogue 2-(4′-hydroxyphenylazo)benzoic acid to be retained. The polymer layers contributed to improving stability of the protein film.

本文言語English
ページ(範囲)361-364
ページ数4
ジャーナルMacromolecular Bioscience
2
8
DOI
出版ステータスPublished - 2002 12 1

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生体材料
  • ポリマーおよびプラスチック
  • 材料化学

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