Preparation of a specifically tritiated locust adipokinetic hormone analog with full biological potency

Koji Muramoto, J. Ramachandran, Pnina Moshitzky, S. W. Applebaum

    研究成果: Article査読

    9 被引用数 (Scopus)

    抄録

    A synthetic peptide related to locus adipokinetic hormone (AKH) and shrimp red pigment concentrating hormone (RPCH) containing a tyrosine residue in place of phenylalanine was iodinated and the 3,5‐diiodotyrosyl derivative was isolated by reverse phase HPLC. Catalytic dehalogenation of the diiodo derivative in the presence of tritium yielded the tritiated AKH analog which was isolated by gel filtration on Sephadex LH‐20 and reverse phase HPLC. The tritiated peptide was formed to be identical to AKH in its ability to stimulate lipid release into the hemolymph of locusts in vivo where the diiodotryrosyl derivative was inactive. The specific radioactivity of the tritiated peptide was 57.2 Ci/mmol, or 99% of the theoretical value.

    本文言語English
    ページ(範囲)443-446
    ページ数4
    ジャーナルInternational Journal of Peptide and Protein Research
    23
    4
    DOI
    出版ステータスPublished - 1984 4

    ASJC Scopus subject areas

    • Biochemistry

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