TY - JOUR
T1 - Preparation and Characterization of Photoactivable Heterobifunctional Fluorescent Reagents
AU - Muramoto, Koji
AU - Kamiya, Hisao
N1 - Funding Information:
Acknowledgments. We are grateful to Dr. J. Ramachandran, University of California, for his guidance in photoaffinity labeling techniques and Dr. Hiroshi Meguro, Tohoku University, for the microanalyses. This work was supported by a Gran-in-Aid for Scientific Research from the Ministry of Education, Science and Culture of Japan.
PY - 1984/1
Y1 - 1984/1
N2 - Two new photoactivable heterobifunctional reagents, l-azido-5-naphthalene sulfonyl chloride (ANS-C1) and the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate (NHS-ANS-AP), were synthesized and characterized. The reagents were applicable to the group-directed modification of ligands. Upon exposure to ultraviolet light, the non-fluorescent modified ligands generated reactive nitrene intermediates which could react with neighboring molecules to form fluorescent products. This was demonstrated by the covalent modification of bovine serum albumin with ANS-AP by photolysis. NHS-ANS-AP was used for the preparation of a photore-active invertebrate lectin. Abbreviations: ANS-C1, l-azido-5-naphthalene sulfonyl chloride; NHS-ANS-AP, the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate; BSA, bovine serum albumin; BRA, Megabalanus rosa lectin; SDS, sodium dodecyl sulfate.
AB - Two new photoactivable heterobifunctional reagents, l-azido-5-naphthalene sulfonyl chloride (ANS-C1) and the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate (NHS-ANS-AP), were synthesized and characterized. The reagents were applicable to the group-directed modification of ligands. Upon exposure to ultraviolet light, the non-fluorescent modified ligands generated reactive nitrene intermediates which could react with neighboring molecules to form fluorescent products. This was demonstrated by the covalent modification of bovine serum albumin with ANS-AP by photolysis. NHS-ANS-AP was used for the preparation of a photore-active invertebrate lectin. Abbreviations: ANS-C1, l-azido-5-naphthalene sulfonyl chloride; NHS-ANS-AP, the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate; BSA, bovine serum albumin; BRA, Megabalanus rosa lectin; SDS, sodium dodecyl sulfate.
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U2 - 10.1271/bbb1961.48.2695
DO - 10.1271/bbb1961.48.2695
M3 - Article
AN - SCOPUS:85007677817
VL - 48
SP - 2695
EP - 2699
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 11
ER -