Preparation and Characterization of Photoactivable Heterobifunctional Fluorescent Reagents

Koji Muramoto, Hisao Kamiya

    研究成果: Article査読

    抄録

    Two new photoactivable heterobifunctional reagents, l-azido-5-naphthalene sulfonyl chloride (ANS-C1) and the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate (NHS-ANS-AP), were synthesized and characterized. The reagents were applicable to the group-directed modification of ligands. Upon exposure to ultraviolet light, the non-fluorescent modified ligands generated reactive nitrene intermediates which could react with neighboring molecules to form fluorescent products. This was demonstrated by the covalent modification of bovine serum albumin with ANS-AP by photolysis. NHS-ANS-AP was used for the preparation of a photore-active invertebrate lectin. Abbreviations: ANS-C1, l-azido-5-naphthalene sulfonyl chloride; NHS-ANS-AP, the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate; BSA, bovine serum albumin; BRA, Megabalanus rosa lectin; SDS, sodium dodecyl sulfate.

    本文言語English
    ページ(範囲)2695-2699
    ページ数5
    ジャーナルAgricultural and Biological Chemistry
    48
    11
    DOI
    出版ステータスPublished - 1984 1

    ASJC Scopus subject areas

    • Biochemistry, Genetics and Molecular Biology(all)
    • Agricultural and Biological Sciences(all)

    フィンガープリント 「Preparation and Characterization of Photoactivable Heterobifunctional Fluorescent Reagents」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル