Preparation and Characterization of Photoactivable Heterobifunctional Fluorescent Reagents

Koji Muramoto, Hisao Kamiya

    研究成果: Article査読

    抄録

    Two new photoactivable heterobifunctional reagents, l-azido-5-naphthalene sulfonyl chloride (ANS-C1) and the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate (NHS-ANS-AP), were synthesized and characterized. The reagents were applicable to the group-directed modification of ligands. Upon exposure to ultraviolet light, the non-fluorescent modified ligands generated reactive nitrene intermediates which could react with neighboring molecules to form fluorescent products. This was demonstrated by the covalent modification of bovine serum albumin with ANS-AP by photolysis. NHS-ANS-AP was used for the preparation of a photore-active invertebrate lectin. Abbreviations: ANS-C1, l-azido-5-naphthalene sulfonyl chloride; NHS-ANS-AP, the N-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate; BSA, bovine serum albumin; BRA, Megabalanus rosa lectin; SDS, sodium dodecyl sulfate.

    本文言語English
    ページ(範囲)2695-2699
    ページ数5
    ジャーナルAgricultural and Biological Chemistry
    48
    11
    DOI
    出版ステータスPublished - 1984 1月

    ASJC Scopus subject areas

    • 生化学、遺伝学、分子生物学(全般)
    • 農業および生物科学(全般)

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