Preparation and characterization of photoactivable heterobifunctional fluorescent reagents

Koji Muramoto, Hisao Kamiya

    研究成果: Article査読

    9 被引用数 (Scopus)

    抄録

    Two new photoactivable heterobifunctional reagents, l-azido-5-naphthalene sulfonyl chloride (ANS-Cl) and the A-hydroxysuccinimide ester of l-azido-5-naphthalene sulfonyl aminopropionate (NHS-ANS-AP), were synthesized and characterized. The reagents were applicable to the group- directed modification of ligands. Upon exposure to ultraviolet light, the non-fluorescent modified ligands generated reactive nitrene intermediates which could react with neighboring molecules to form fluorescent products. This was demonstrated by the covalent modification of bovine serum albumin with ANS-AP by photolysis. NHS-ANS-AP was used for the preparation of a photoreactive invertebrate lectin.

    本文言語English
    ページ(範囲)2695-2699
    ページ数5
    ジャーナルAgricultural and Biological Chemistry
    48
    11
    DOI
    出版ステータスPublished - 1984 11

    ASJC Scopus subject areas

    • 生化学、遺伝学、分子生物学(全般)
    • 農業および生物科学(全般)

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