Preparation and characterization of agonistic monoclonal antibodies against toll-like receptor 4-MD-2 complex

Uleng Bahrun, Masao Kimoto, Hiroki Tsukamoto, Naoko Tsuneyoshi, Jun Kohara, Kenji Fukudome

研究成果: Article査読

15 被引用数 (Scopus)

抄録

Ligands for toll-like receptors (TLR) are known to induce a variety of immune responses. Selective induction of desirable responses would be important for the treatment of individual diseases with various pathogenesis. For this purpose, we established six MAbs against the TLR4/MD-2 complex (UT MAbs) from TLR4-/- mice or MD-2-/- mice. Three MAbs (UT12, 18, and 22) induced NF-κB activation and production of pro-inflammatory cytokines, but the other three (UT15, 41, and 49) did not induce such cell responses. Unlike lipopolysaccharide (LPS), agonistic UT MAbs did not require serum components for the functions. UT41 and UT49 recognized TLR4 in the absence of MD-2. On the other hand, the other four MAbs reacted to the TLR4/MD-2 complex, but not to solo TLR4. Agonistic UT MAbs shared the epitopes, but non-agonistic UT15 reacted to distinct epitope on the complex. UT MAbs appear to be useful analyzing the molecular mechanism of TLR signaling and will contribute to the development of novel immunotherapies.

本文言語English
ページ(範囲)393-399
ページ数7
ジャーナルHybridoma
26
6
DOI
出版ステータスPublished - 2007 12 1

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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