Practical synthesis of maleimides and coumarin-linked probes for protein and antibody labelling via reduction of native disulfides

Hong Y. Song, Mun H. Ngai, Zhen Y. Song, Paul A. MacAry, Jonathan Hobley, Martin J. Lear

研究成果: Article査読

74 被引用数 (Scopus)

抄録

The cellular tracking, detection and sensing of protein or antibody movement are important aspects to advance our understanding of biomolecular interactions and activity. Antibodies modified with fluorescent dyes are also valuable tools, especially in immunology research. We describe here a proof-of-principle study of a new water-soluble coumarin probe with a maleimide thiol-reacting unit to fluorescently tag biomolecules. Highlights include: (1) a convenient water-based preparation of N-substituted maleimides, (2) a one-pot preparation of activated maleimido-esters, and (3) a bio-conjugation protocol for the selenol-promoted reduction of native disulfide bonds and the 'site-specific' labelling of antibodies with no significant loss of activity.

本文言語English
ページ(範囲)3400-3406
ページ数7
ジャーナルOrganic and Biomolecular Chemistry
7
17
DOI
出版ステータスPublished - 2009
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 物理化学および理論化学
  • 有機化学

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