Potentiation of nitric oxide formation following bilateral carotid occlusion and focal cerebral ischemia in the rat: in vivo detection of the nitric oxide radical by electron paramagnetic resonance spin trapping

Teiji Tominaga, Shinya Sato, Tomoko Ohnishi, S. Tsuyoshi Ohnishi

研究成果: Article査読

107 被引用数 (Scopus)

抄録

We have directly demonstrated in vivo that nitric oxide (NO) is produced in the ischemic rat brain. Using diethyldithiocarbamate and Fe as spin-trapping agents, NO spin adducts were detected by cryogenic electron paramagnetic resonance. The cerebral cortex which was exposed to focal ischemia or bilateral carotid artery occlusion generated an increased amount of spin-adducts of NO radicals (g = 2.039, a hyperfine coupling constant aN = 13 gauss). This signal disappeared by the preischemic administration of NG-nitro-l-arginine methylester, a NO synthase inhibitor.

本文言語English
ページ(範囲)342-346
ページ数5
ジャーナルBrain research
614
1-2
DOI
出版ステータスPublished - 1993 6月 18
外部発表はい

ASJC Scopus subject areas

  • 神経科学(全般)
  • 分子生物学
  • 臨床神経学
  • 発生生物学

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