Positive and negative regulation of the human heme oxygenase-1 gene expression in cultured cells

Shigeru Takahashi, Yuji Takahashi, Kazumi Ito, Tadahiro Nagano, Shigeki Shibahara, Takashi Miura

研究成果: Article査読

33 被引用数 (Scopus)

抄録

To elucidate the regulation of the human heme oxygenase-1 (hHO-1) gene expression, we assessed approximately 4 kb of the 5'-flanking region of the hHO-1 gene for basal promoter activity and sequenced approximately 2 kb of the 5'-flanking region. A series of deletion mutants of the 5'-flanking region linked to the luciferase gene was constructed. Basal level expression of these constructs was tested in HepG2 human hepatoma cells and HeLa cervical cancer cells. By measuring luciferase activity, which was transiently expressed in the transfected cells, we found a positive regulatory region at position -1976 to -1655 bp. This region functions in HepG2 cells but not in HeLa cells. A negative regulatory region was also found at position -981 to -412 bp that functions in both HepG2 cells and HeLa cells. Copyright (C) 1999 Elsevier Science B.V.

本文言語English
ページ(範囲)231-235
ページ数5
ジャーナルBiochimica et Biophysica Acta - Gene Structure and Expression
1447
2-3
DOI
出版ステータスPublished - 1999 10 28
外部発表はい

ASJC Scopus subject areas

  • 構造生物学
  • 生物理学
  • 生化学
  • 遺伝学

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