Polyphasic identification of a thermophilic bacterium from geyser of Cisolok, Indonesia

Abinubli Tariswafi Mawarid, Wellyzar Sjamsuridzal, Shuhei Yabe, Iman Santoso, Fitria Ningsih, Dafina Ghossani Nurlaili, Ariyanti Oetari, Akira Yokota

研究成果: Conference contribution

3 被引用数 (Scopus)

抄録

A thermophilic bacterium strain UICC B-76 was isolated from litter in geyser of Cisolok, West Java, Indonesia by using The International Streptomyces Project (ISP1) medium. The strain UICC B-76 was able to hydrolyze various substrates such as casein, cellulose, gelatin, starch, and xylan, at 50 °C, and decolorize Remazol Brilliant Blue R (RBBR) at 50 °C, an indicator for ligninolytic degradation ability. These abilities of strain UICC B-76 suggest its potential for industrial applications. The aim of this study was to identify strain UICC B-76 based on phenotypic and genotypic characterizations. Strain UICC B-76 was Gram-positive, spore-forming, aerobic and facultative anaerobic, and rod-shaped with flagella. The strain grew at 30 °C to 60 °C (optimum 50 °C) and pH 7.0 but can not withstand temperature of 70 °C as well as pH 4.0 and 9.0. Strain UICC B-76 could not grow in more than 1% NaCl. This strain has menaquinone 7 (MK-7) as the major quinone system; cell wall fatty acids consisted of Iso-C15:0 (68.56%), Iso-C17:0 (12.48%), and Anteiso-C15:0 (8.45%); and the main amino acids of the cell wall was meso-diaminopimelic acid (meso-DAP). Phylogenetic analysis based on the full sequence of 16S rRNA gene (1450 nt) indicated that strain UICC B-76 belongs to the genus Brevibacillus (Family Paenibacillaceae, Order Bacillales, Class Bacilli, Phylum Firmicutes). Strain UICC B-76 was closely related with type strains of Brevibacillus parabrevis and those of B. agri, however, strain UICC B-76 was separated from both species. The 16S rDNA sequence similarity of strain UICC B-76 showed relatively low sequence homology value, less than 99%, to the type of strains B. parabrevis (ranged between 98.13% and 98.20%) and B. agri (98.13%). Moreover, the G+C content of strain UICC B-76 (55 mol%) differs with B. parabrevis (51.8-52.2 mol%) and B. agri (53.5 mol%). This result indicated that strain UICC B-76 may represent a new species of Brevibacillus. Hybridization of DNA among strain UICC B-76, B. parabrevis and B. agri is required to clarify the proposal of a new species.

本文言語English
ホスト出版物のタイトルInternational Symposium on Current Progress in Mathematics and Sciences 2015, ISCPMS 2015
ホスト出版物のサブタイトルProceedings of the 1st International Symposium on Current Progress in Mathematics and Sciences
編集者Terry Mart, Djoko Triyono
出版社American Institute of Physics Inc.
ISBN(電子版)9780735413764
DOI
出版ステータスPublished - 2016 4 19
イベント1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015 - Depok, Indonesia
継続期間: 2015 11 32015 11 4

出版物シリーズ

名前AIP Conference Proceedings
1729
ISSN(印刷版)0094-243X
ISSN(電子版)1551-7616

Other

Other1st International Symposium on Current Progress in Mathematics and Sciences, ISCPMS 2015
国/地域Indonesia
CityDepok
Period15/11/315/11/4

ASJC Scopus subject areas

  • 物理学および天文学(全般)

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